Part:BBa_Z0170:Design

T7.2 gene 1, codon-shuffled

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

The nucleotide sequence of the first 12 AA after the start codon were codon-shuffled to minimize the predicted RNA secondary structure that is formed between the RBS assigned to this gene in the T7.2 design and the coding sequence. The predicted dG of the RBS-CDS junction is -0.01 kCal/mol.

Source

Based on gene 1 of T7.